Home > Sandwich ELISA Kits > Human Monocyte Chemotactic Protein 4 (MCP-4;CCL13) ELISA Kit

Product Name:

Human Monocyte Chemotactic Protein 4 (MCP-4;CCL13) ELISA Kit

Cat.#:

JL11285

Regulatory Status:

RUO

Alternative:

CCL13; SCYA13; NCC1; SCYL1; CKb10; Chemokine C-C-Motif Ligand 13; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 13; CK-beta-10; Monocyte chemoattractant 4

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.62-1000pg/mL

Sensitivity:

7.84pg/mL

Specificity:

This kit recognizes Human MCP-4;CCL13 in samples. No significant cross-reactivity or interference between Human MCP-4;CCL13 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MCP-4;CCL13

Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family.CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5.Activity of this chemokine has been implicated in allergic reactions such as asthma.CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-α.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	1000	500	250	125	62.5	31.25	15.62	0
OD	2.39	1.73	1.2	0.78	0.49	0.35	0.23	0.08
Corrected OD	2.31	1.65	1.12	0.7	0.41	0.27	0.15	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MCP-4;CCL13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	94-105	104
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human MCP-4;CCL13 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	85-108	105-115
1 : 4	Average (%)	94	96	108